Patel , Satya Narayan and Sharma, Manisha and Lata, Kusum and Singh, Umesh and Kumar , Vinod and Sangwan, Rajender S and Singh, Sudhir P (2016) Improved operational stability of d-psicose 3-epimerase by a novel protein engineering strategy, and d-psicose production from fruit and vegetable residues. Bioresource Technology, 216. pp. 121-127. ISSN 0960-8524

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Abstract

The aim of the present work was to improve stability of d-psicose 3-epimerase and biotransformation of fruit and vegetable residues for d-psicose production. The study established that N-terminal fusion of a yeast homolog of SUMO protein – Smt3 – can confer elevated optimal temperature and improved operational stability to d-psicose 3-epimerase. The Smt3-d-psicose 3-epimerase conjugate system exhibited relatively better catalytic efficiency, and improved productivity in terms of space–time yields of about 8.5 kg L−1 day−1. It could serve as a promising catalytic tool for the pilot scale production of the functional sugar, d-psicose. Furthermore, a novel approach for economical production of d-psicose was developed by enzymatic and microbial bioprocessing of fruit and vegetable residues, aimed at epimerization of in situd-fructose to d-psicose. The bioprocessing led to achievement of d-psicose production to the extent of 25–35% conversion (w/w) of d-fructose contained in the sample.

Item Type:	Article
Uncontrolled Keywords:	d-Psicose; Smt3; d-Psicose 3-epimerase; Residual biomass; Fruit and vegetable waste; Bioprocessing
Subjects:	Cell biology Genetics Molecular Biology Computational Biology
Depositing User:	Mr Pqr Xyz
Date Deposited:	05 Aug 2016 05:06
Last Modified:	05 Aug 2016 05:06
URI:	http://ciab.sciencecentral.in/id/eprint/3

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